An Easy and Small-Scale Sample Preparation Technique Followed by HPLC-PDA Analysis for Determining Canthaxanthin in Chicken Liver, Fat, and Egg Yolk
نویسنده
چکیده
This paper described a fast, simple, and small scale method of sample preparation followed by High-Performance Liquid Chromatography (HPLC) coupled Photo-Diode Array (PDA) detector for quantification of canthaxanthin in chicken liver and fat, and hen’s egg yolk. The HPLC-PDA was performed on a C18 column with an isocratic mobile phase. Analyte was extracted from the sample using a handheld ultrasonic homogenizer, and purified by MonoSpin-SI, a centrifugal monolithic silica spin mini-columns, and quantified <15 min. The proposed method obtained average recoveries for the analyte in the range of 93.5-101.0% with relative standard deviations ≤ 2.7%. The quantification limits in chicken liver, fat, and egg yolk were 0.48, 0.47, and 0.5 μg g, respectively.
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